Hampton University MARC Program - Personnel

Dr. Neelam Azad Dr. Neelam Azad Associate Professor and Chair, Pharmaceutical Sciences Location:Flemmie Kittrell Hall1st Floor, Main Office Phone:757-727-5071

Education

Ph.D. (Pharmaceutical Sciences), West Virginia University, 2007

B.S. (Pharmacy), University of Mumbai, 2001

 

Honors & Awards

2015-2016           Academic Leadership Fellows Program (ALFP), American Association of Colleges of Pharmacy

2015, 2014, 2013 Professor of the Year Award, Department of Pharmaceutical Sciences, Hampton University

2014, 2013, 2012 AACR Minority-Serving Institution Faculty Scholar Award, America Association for Cancer Research Annual Conference

2013                    President’s Ambassador for Academic Excellence Award,Hampton University

2013                    Scholarship, Frontiers in Stem Cells in Cancer Workshop, Magee Women’s Research Institute, University of Pittsburgh

2012                    Scholarship, Workshop on Leadership in Bioscience, Cold Spring Harbor Laboratory

2010                    Induction into Rho Chi Honor Society

2010                    Conference Award, Towards Personalized Cancer Medicine Conference, The New York Academy of Sciences

2006                    Research Award in Biotechnology, American Association of Pharmaceutical Scientists Annual Conference

2006                    Induction into Sigma Xi Honor Society

2006                    Sigma Xi Research Award, Chapter’s Annual Research Day, West Virginia University

2006                    Annual Research Award, Department of Pharmaceutical and Pharmacological Sciences, West Virginia University

2006, 2005          Van Liere Research Award,Health Science Center’s Annual Research Day, West Virginia University

2004                    Best Teaching Assistant Award, Department of Pharmaceutical and Pharmacological Sciences, West Virginia University


Grantsmanship

SC1HL112630-01                                                                09/01/2011 - 06/30/2016

National Institute of Health (NIH)/National Heart Lung and Blood Institute

Title: Impact of Oxidative Stress-Mediated Angiogenesis in Pulmonary Fibrosis

Role: Principal Investigator            Amount: $ 1.3 Million

 

SC1CA173069-01A1                                                            09/01/2012 - 06/30/2017

NIH/National Cancer Institute (NCI)

Title: Anti-Tumorigenic Potential of Novel Digitoxin Analogues

Role: Co-investigator                      Amount: $ 1.35 Million

 

Research Interests

  • Fibrosis – Genetic and molecular networks involved in pulmonary fibrosis.
  • Cancer – Molecular mechanisms involved in cancer progression and metastasis.
  • Apoptosis – Effect of environmental and occupational carcinogens on apoptosis.
  • Free Radical Biology – Oxidative and nitrosative stress in cancer and fibrosis.
  • Nanotoxicology – Toxicologic assessment of various nanoparticles.

 

Publications

SCIENTIFIC PAPERS

  • Yakisich J.S., Venkatadri R., Neelam Azad, Iyer A.K.V. Chemoresistance of Lung and Breast Cancer Cells Growing Under Prolonged Periods of Serum Starvation. Journal of Cellular Physiology (2016). [Epub ahead of print]
  • Wright C. A., Iyer A.K.V, Wang L., Wu N., Yakisich J.S., Rojanasakul Y., Neelam Azad. Effects of titanium dioxide nanoparticles on human keratinocytes. Drug Chem Toxicol. (2016), 16, 1-11. 
  • Venkatadri R., Muni T., Iyer A.K.V., Yakisich J.S., Neelam Azad. Role of Apoptosis-Related MiRNAs in Resveratrol-Induced Breast Cancer Cell Death.  Cell Death and Disease (2016). (Epub ahead of print)
  • Klionsky D.J., Neelam Azad et al. Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy (2016), 12 (1): 1-222.
  • Yakisich J.S., Neelam Azad, Venkatadri R., Kulkarni Y., Wright C., Kaushik V., O’Doherty G., Iyer A.K.V. Digitoxin and its Synthetic Analog MonoD have Potent Antiproliferative Effects on Lung Cancer Cells and Potentiate the Effects of Hydroxyurea and Paclitaxel. Oncology Reports (2016), 35(2), 878-86. 
  • Wright C. A., Kulkarni Y. M., Iyer A.K.V, Neelam Azad. S-nitrosylation of Bcl-2 Negatively Affects Autophagy in Lung Epithelial Cells. Journal of Cellular Biochemistry (2016), 117(2), 521-32. 
  • Yakisich J.S., Neelam Azad, Venkatadri R., Kulkarni Y., Wright C., Kaushik V., Iyer A.K.V. Formation of Tumorspheres with Increased Stemness without External Mitogens in a Lung Cancer Model. Stem Cells International (2016).Volume 2016, Article ID 5603135, 6 pages (Open Access Article).URL: http://dx.doi.org/10.1155/2016/5603135
  • Kulkarni Y. M., Dutta S., Iyer A.K.V, Venkatadri R., Kaushik V., Ramesh V., Wright C. A., Semmes O.J., Yakisich J.S., Neelam Azad. A Proteomics Approach to Identifying Key Protein Targets Involved in VEGF-Inhibitor Mediated Attenuation of Bleomycin-Induced Pulmonary Fibrosis. Proteomics (2016), 16(1), 33-46.
  • Iyer A.K.V, Ramesh V., Castro C.A., Kaushik V., Kulkarni Y. M., Wright C. A., Venkatadri R., Rojanasakul Y., Neelam Azad. Nitric Oxide Mediates Bleomycin-Induced Angiogenesis and Pulmonary Fibrosis via Regulation of VEGF. Journal of Cellular Biochemistry (2015), 116(11), 2484-93
  • Kulkarni Y., Kaushik V., Neelam Azad, Wright C., Rojanasakul Y., O’Doherty G., Iyer A.K.V.  Autophagy-Induced Apoptosis in Lung Cancer cells by a Novel Digitoxin Analog. Journal of Cellular Physiology (2015) (Epub ahead of Print)
  • Iyer A.K.V, Ramesh V., Castro C.A., Kaushik V., Kulkarni Y. M., Wright C. A., Venkatadri R., Rojanasakul Y., Neelam Azad. Nitric Oxide Mediates Bleomycin-Induced Angiogenesis and Pulmonary Fibrosis via Regulation of VEGF. Journal of Cellular Biochemistry (2015). [Epub ahead of print doi:10.1002/jcb.25192]
  • Talbott S.J., Luanpitpong S., Stehlik C., Neelam Azad, Iyer A.K.V., Wang L., Rojanasakul Y. S-Nitrosylation of FLICE Inhibitory Protein Determines its Interaction with RIP1 and Activation of NF-kB. Cell Cycle (2014), 13(12), 948-957.
  • Neelam Azad, Iyer A.K.V., Lu Y., Wang L., Rojanasakul Y. P38/MAPK Regulates Single-walled Carbon Nanotube-Induced Fibroblast Proliferation and Collagen Production. Nanotoxicology (2013),7(2), 157–168.
  • Luanpitpong S., Iyer A.K.V., Neelam Azad, Wang L., Rojanasakul Y. Nitrosothiol Signaling in Anoikis Resistance and Cancer Metastasis. Forum on Immunopathological Diseases and Therapeutics (2012),3(2), 141–154.
  • Djordje M., Luanpitpong S., Neelam Azad, Wang L., Jiang B. H., Davis M., Barnett J., Guo L., Rojanasakul Y. Multifunctional Role of Bcl-2 in Malignant Transformation and Tumorigenesis of Cr(VI)-Transformed Lung Cells. PLoS ONE (2012), 7(5), e37045.
  • Iyer A.K.V., Neelam Azad, Wang L., and Rojanasakul Y. Cellular FLICE-Inhibitory Protein Regulates Fas-Ligand Induced Activation of NFκB in A PI3K-Dependent Manner. Journal of Immunology (2011), 187(6), 3256-66.
  • Iyer A.K.V., Zhou M., Neelam Azad, Elbaz, H., Wang H., Rogalsky D., Rojanasakul Y., O’Doherty G. and Langenhan J. A Direct Comparison of the Anticancer Activities of Digitoxin MeON-Neoglycosides and O-Glycosides: Oligosaccharide Chain Length Dependant Induction of Caspase-9-Mediated Apoptosis. American Chemical Society Medicinal Chemistry Letters (2010), 1(7), 300-369.
  • Neelam Azad, Iyer A.K.V., Wang L., Lu Y., Medan D., Castranova V., Rojanasakul Y. Nitric Oxide-Mediated Bcl-2 Stabilization Potentiates Malignant Transformation of Human Lung Epithelial Cells. American Journal of Respiratory Cellular and Molecular Biology (2010), 42 (5), 578-585.
  • Neelam Azad, Lu Y., Wang L., Iyer A.K.V., Castranova V., Jiang B.H., Rojanasakul Y. Phosphatidylinositol-3-Kinase/Akt Regulates Bleomycin-Induced Fibroblast Proliferation and Collagen Production. American Journal of Respiratory Cellular and Molecular Biology (2010), 42 (4), 432-441.
  • Neelam Azad, Iyer A.K.V., Manosroi A., Wang L., Rojanasakul Y. Superoxide-Mediated Proteasomal Degradation of Bcl-2 Determines Cell Susceptibility to Cr(VI)-Induced Apoptosis. Carcinogenesis (2008), 29(8), 1538-1545.
  • Iyer, A.K.V, Neelam Azad, Rojanasakul, Y. Role of S-nitrosylation in Apoptosis Resistance and Malignant Transformation. Nitric Oxide Biology and Chemistry (2008), 19(2), 146-151.
  • Kongkaneramit L., Sarisuta N., Neelam Azad, Lu Y., Iyer A.K.V., Wang L., Rojanasakul Y. Dependence of Reactive Oxygen Species and FLIP on Lipofectamine-Induced Apoptosis in Human Lung Epithelial Cells. Journal of Pharmacology and Experimental Therapeutics (2008), 325(3), 969-977.
  • Wang L., Neelam Azad, Kongkaneramit L., Chen F., Lu Y., Jiang B.H., Rojanasakul Y. The Fas Death Signaling Pathway Connecting Reactive Oxygen Species Generation and FLICE Inhibitory Protein Downregulation. Journal of Immunology (2008), 180(5), 3072-3080.
  • Moungjaroen J., Nimmannit U., Callery P.S., Wang L., Neelam Azad, Lipipun V., Chanvorachote P., Rojanasakul Y. Reactive Oxygen Species Mediate Caspase Activation and Apoptosis Induced by Lipoic Acid in Human Lung Epithelial Cancer Cells through Bcl-2 Downregulation. Journal of Pharmacology and Experimental Therapeutics (2006), 319(3), 1062-1069.
  • Neelam Azad, Vallyathan V., Wang L., Tantishaiyakul V., Stehlik C., Leonard S.S., Rojanasakul Y. S-nitrosylation of Bcl-2 inhibits its ubiquitin-proteasomal degradation: A novel anti-apoptotic mechanism that suppresses apoptosis. The Journal of Biological Chemistry (2006), 281(45), 34124-34134.
  • Chanvorachote P., Nimmannit U., Wang L., Stehlik C., Lu B., Neelam Azad, Rojanasakul Y. Nitric Oxide Negatively Regulates Fas CD95-induced Apoptosis through Inhibition of Ubiquitin-Proteasome mediated Degradation of FLICE Inhibitory Protein. The Journal of Biological Chemistry (2005), 280(51), 42044–42050.


SCIENTIFIC REVIEWS

  • Iyer, A.K.V, Neelam Azad, Rojanasakul, Y. Nitrosothiol Signaling and Protein Nitrosation in Cell Death and    Survival. Nitric Oxide Biology and Chemistry (2014), 42C, 9-18. 

  • Neelam Azad, Iyer A.K.V., Vallyathan V., Wang L., Castranova V., Stehlik C., Rojanasakul Y. Role of Oxidative/Nitrosative Stress-Mediated Bcl-2 Regulation in Apoptosis and Malignant Transformation. Annals of The New York Academy of Sciences (2010), 1203, 1-6.

  • Neelam Azad, Rojanasakul, Y. and Vallyathan, V. Inflammation and Lung Cancer: Roles of Reactive Oxygen/Nitrogen Species. Journal of Toxicology and Environmental Health, Part B (2008), 11(1), 1-15.

  • Neelam Azad, Rojanasakul Y. Nanobiotechnology in Drug Delivery. American Journal of Drug Delivery (2006), 4(2), 79-88.

  • Neelam Azad, Rojanasakul Y. Vaccine Delivery - Current Trends and Future. Current Drug Delivery (2006), 3(2), 137-146.

 

BOOK CHAPTERS

  • Neelam Azad, Iyer A. K. V. Reactive Oxygen Species and Apoptosis. Systems Biology of Free Radicals and Antioxidants (2014), 113-135.
  • Iyer, A.K.V, Neelam Azad, Rojanasakul Y, Ma J.K.H. Application of Nanosciences in Pharmaceutics. Basic Physical Pharmacy (2012), 486-516.

  • Iyer, A.K.V, Neelam Azad, Rojanasakul, Y. S-nitrosylation – How Cancer Cells Say NO to Cell Death. Nitric Oxide Biology and Chemistry, Prognosis, Prevention and Therapy (2010), Part 3, 4-102.

  • Neelam Azad, Iyer A. K. V., Rojanasakul Y. Methods to Analyze S-nitrosylation of Proteins Involved in Apoptosis. Methods in Molecular Biology (2009), 559, 117-130.

  • Neelam Azad, Iyer A. K. V., Rojanasakul Y. DNA Microarrays in Drug Discovery and Development. Biopharmaceutical Drug Design and Development (2007), 2nd Edition, 47-66.

  • Neelam Azad, Rojanasakul Y. Macromolecular Drug Delivery. Biopharmaceutical Drug Design and Development (2007), 2nd Edition, 293-324.

     

Selected Presentations

  • Venkatadri R., Wright C., Kaushik V., Kulkarni Y., Yakisich J.S., Iyer A.K.V, Neelam Azad. MnTBAP Inhibits Bleomycin-Induced Pulmonary Fibrosis by Regulating Canonical Wnt Pathway. PFF Summit: From Bench to Bedside, Pulmonary Fibrosis Foundation Annual Conference, 2015.(Poster)
  • Wright C., Iyer A.K.V, Neelam Azad. Differential autophagy responses in non-tumorigenic and malignantly transformed lung epithelial cells. AACR Annual Conference,2014.(Poster)
  • Neelam Azad, Rojanasakul Y., O’ Doherty G., Iyer A.K.V. Anti-tumorigenic Effects of a Novel Digitoxin Analogue. AACR Annual Conference, 2013. (Poster)
  • Neelam Azad. Role of Oxidative Stress Mediated Angiogenesis in Pulmonary Fibrosis. Graduate Seminar series, College of Pharmacy, Nova Southeastern University, 2012.(Podium)
  • Neelam Azad, Iyer A.K.V., Wang L., Y Lu., Rojanasakul Y. Impact of Oxidative Stress-Regulated Angiogenesis in Pulmonary Fibrosis. PFF Summit 2011: From Bench to Bedside, Inaugural Conference of Pulmonary Fibrosis Foundation, 2011. (Poster)
  • Neelam Azad. Molecular Mechanisms in Hexavalent Chromium-Induced Lung Pathogenesis. 6th Conference on Molecular Mechanisms of Metal Toxicity and Carcinogenesis. Sponsored by the National Institute for Environmental Health Sciences and Health and the University of Kentucky, 2010. (Podium)
  • Neelam Azad, Iyer A.K.V., Rojanasakul Y. Malignant Transformation of Human Lung Epithelial Cells – Interplay Between Nitric Oxide, Bcl-2 And VEGF. Towards Personalized Cancer Medicine, NYAS, 2010. (Poster)
  • Neelam Azad. Reactive Oxidative/Nitrosative Species in Lung Cancer and Fibrosis. 4th International Conference on Oxidative/Nitrosative Stress and Disease. The New York Academy of Sciences (NYAS), 2009. (Podium)
  • Neelam Azad, Iyer A.K.V., Lu Y., Wang L., Rojanasakul Y. Angiogenic Mediators Regulate Single-Walled Carbon Nanotube-Induced Fibrogenesis in Human Lung Fibroblasts. AAPS, 2008. (Poster)
  • Neelam Azad, Wang L., Rojanasakul Y. Reactive Oxygen Species Mediate Apoptosis in Human Lung Epithelial Cancer Cells through Ubiquitin-Mediated Bcl-2 Downregulation. AAPS, 2007. (Poster)
  • Neelam Azad. S-Nitrosylation of Bcl-2 - A Novel Anti-Apoptotic Mechanism. American Association of Pharmaceutical Scientists (AAPS), 2006. (Podium)

 

Professional Affiliations

  • Rho Chi Pharmacy Honor Society
  • Sigma Xi Research Honor Society
  • American Association of Cancer Research (AACR)
  • Minorities in Cancer Research (MICR)
  • American Association of Colleges of Pharmacy (AACP)

Have Questions?

Please contact Dr. Cecile Andraos-Selim, HU MARC Program Director, Associate Professor of Biology, Department of Biological Sciences; DU117; 757-727-5015; cecile.andraos-selim@hamptonu.edu

The MARC Program is Funded by NIH/NIGMS Grant #1T34GM105550-01A1